1 Optimization of the pepsin digestion method for anisakis inspection in the fish industry 1 2
نویسندگان
چکیده
17 18 During the last 50 years human anisakiasis has been rising while parasites have increased their prevalence at 19 determined fisheries becoming an emergent major public health problem. Although artificial enzymatic digestion 20 procedure by CODEX (STAN 244-2004: Standard for salted Atlantic herring and salted sprat) is the recommended 21 protocol for anisakids inspection, no international agreement has been achieved in veterinary and scientific digestion 22 protocols to regulate this growing source of biological hazard in fish products. The aim of this work was to optimize the 23 current artificial digestion protocol CODEX with the purpose of offering a faster, more useful and safety procedure than 24 the current one for anisakids detection. To achieve these objectives, the existing pepsin chemicals and the conditions of 25 the digestion method were evaluated and assayed in fresh and frozen samples, both in lean and fatty fish species. New 26 conditions were introduced with the objective of being tested, thus improving the current digestion protocol. Results 27 showed that the new digestion procedure considerably reduces the assay time, and it is more handy and efficient (the 28 quantity of the resulting residue was considerably lower after less time) than the largely used from CODEX STAN 24429 2004. In conclusion, the new digestion method herein proposed based on liquid pepsin format, is an accurate 30 Manuscript
منابع مشابه
The accuracy of visual inspection for preventing risk of Anisakis spp . infection in unprocessed fish 1 2
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